Use of Prussian Blue/Conducting Polymer Modified Electrodes for the Detection of Cytochrome C

In this work, the development and utilization of a Prussian blue/conducting polymer composite electrode has been investigated. The introduction of a layer of conducting polymer (polypyrrole) enhanced the electroactivity of the outer Prussian blue film. This has been used for improved signal generation for the redox protein cytochrome c. The effect of the thickness of these two film coatings and buffer solution pH has been investigated. The analytical utility has been demonstrated using flow injection analysis.