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Ever since the introduction of immunohistochemical staining (IHC), histotechnologists around the world have been faced with a growing demand for faster and more accurate staining (maximum antigen detection with minimum nonspecific background staining). Viewing the different techniques developed, combined with the numerous ‘traps’ a specimen holds, IHC staining today is a real challenge. Facing this challenge, it may be helpful to give an overview of the different choices a technician has within IHC staining. When answering todays call for standardization, or stepping into automation, it is essential to analyze the different steps involved (from fixation to mounting) in order to respond to possible errors or problems. This article provides this basic information, viewed from a routine European anatomic pathology lab. (The J Histotechnol 25:45, 2002)
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Donald Van Hecke (2002) studied this question.
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