A miniaturised restriction enzyme analysis method for HSV typing offers comparable accuracy to standard methods with improved safety and lower cost for routine laboratory use.
May support safer, lower-cost HSV typing in routine labs; leaves open independent validation before practice change.
A miniaturised, simplified and rapid method for typing and subtyping herpes simplex virus by restriction enzyme analysis of radioactively labelled DNA is described. This method requires far less label than those described previously, giving advantages in both cost and safety and therefore in its suitability for use in a routine laboratory. It produces results indistinguishable from those obtained by the parent method.
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J. M. Darville (1983) studied this question.
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