Seeing through Macromolecules: T₂-Filtered NMR for the Purity Assay of Functionalized Nanosystems and the Screening of Biofluids

For samples whose NMR spectra are dominated by the broad signals of macromolecules or functionalized nanoparticles, transverse relaxation (T(2)) spectral editing lends itself to a precise identification and determination of small molecules such as metabolites or contaminants. In order to retain the most sensitivity, we propose a method for efficiently removing the interference of spin-spin couplings that typically lead to signal losses in standard pulse schemes designed for T(2) editing.