Identifying key perturbations of redox regulatory mechanisms may help to understand stress-related disease processes.Currently, flow cytometry allows the functional characterisation of redox changes that occur in different cellular compartments: changes in mitochondrial membrane potential, respiratory burst activity, and intracellular thiol levels are measurable in live white blood cells.However, flow cytometric techniques generally require leukocyte fraction isolation, followed by re-suspension and counting.The purpose of the study was to develop a rapid cytofluorimetric assay to measure the redox triplet in peripheral leukocytes without preliminary isolation.
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Matteucci et al. (2009) studied this question.
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