April 3, 1990Biochemistry

Interaction of myosin subfragment 1 with fluorescent ribose-modified nucleotides. A comparison of vanadate trapping and SH1-SH2 crosslinking

View Full Paper

Ask AI

Bookmark

Population

Skeletal myosin subfragment 1 (S1)

Comparison

Fluorescent ribose-modified nucleotides with… vs Reversibly bound S1-nucleotide complexes

Design

Preclinical

Authors

CCChristine CremoUniversity of Nevada, Reno JNJoan M. Neuron RYRalph G. YountWashington State University

Discussion

Member takes

Implication

Offers no immediate clinical implications for myosin-targeted therapies; leaves open conformational dynamics in cardiac myosin models.

Structured PICO

Population

Skeletal myosin subfragment 1 (S1)

Intervention

Fluorescent ribose-modified nucleotides (DEDA-ADP and MANT-ADP) with vanadate (Vi) trapping or N,N'-p-phenylenedimaleimide (pPDM) thiol cross-linking

Comparator

Reversibly bound S1-nucleotide complexes

Outcome

Steady-state and time-resolved fluorescent properties (quantum yield, lifetime, polarization, solute accessibility)surrogate

Trapping of skeletal myosin subfragment 1 by pPDM or vanadate induces a conformational change near the ribose binding site that relieves static quenching of fluorescent nucleotide analogues.

Cite This Study

Cremo et al. (1990) studied this question.

synapsesocial.com/papers/6a71432026a7f98052dda4b8 https://doi.org/10.1021/bi00465a023

View Full Paper

Ask AI

Bookmark

Interaction of myosin subfragment 1 with fluorescent ribose-modified nucleotides. A comparison of vanadate trapping and SH1-SH2 crosslinking

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Interaction of myosin subfragment 1 with fluorescent ribose-modified nucleotides. A comparison of vanadate trapping and SH1-SH2 crosslinking

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider