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A flavoprotein, named azotoflavin, was isolated from an extract of Azotobacter vinelandii cells, which linked the reducing power generated by illuminated spinach chloroplasts to the Azotobacter nitrogen-fixing enzyme complex. The photoreduction of the yellow azotoflavin by chloroplasts produced a stable, free-radical semiquinone, blue in color, with properties similar to those described by other investigators for an Azotobacter flavoprotein of unknown biological function.
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Benemann et al. (1969) studied this question.
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