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Extracts prepared from sporulating cultures of Bacillus megaterium can carry out the net synthesis of dipicolinic acid when supplemented with pyruvate and aspartic semialdehyde. The dipicolinic acid-synthesizing system cannot be detected in extracts prepared from vegetative cells. Its induction in the sporulating cultures corresponds, in time, to the period of dipicolinic acid synthesis in vivo.
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Bach et al. (1966) studied this question.
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