Hydrogen peroxide reacts with ferrous horseradish peroxidase and converts it to oxyperoxidase in a sequence of two reactions. The first is the reaction of ferrous peroxidase with H2O2 to form Compound II; the second is the reaction of Compound II with H2O2 to form oxyperoxidase. Both reactions follow second order kinetics, being first order with respect to each of the reactants. They proceed without detectable intermediates, and, therefore, appear to be single step 2-electron oxidations.
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Noble et al. (1970) studied this question.
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