Isolation of temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine

Temperature-sensitive mutants were isolated from a tetracycline resistance plasmid, pSC101, after mutagenesis with hydroxylamine. They were divided into three classes according to their growth characteristics. They include mutants defective in the replication of plasmid deoxyribonucleic acid and strains having a mutation in the drug resistance gene.