Large-field optical sectioning structured illumination microscopy

Nowadays, fringe generation and fast-switching in structured illumination microscopy (SIM) is often performed using spatial light modulators (SLMs) and digital micromirror devices (DMDs). Yet, the field of view (FOV) or the spatial bandwidth product (SBP) is constrained by the limited pixel number of the SLMs or DMDs. In this study, we present a large-field optical-sectioning structured illumination microscopy (LF-OS-SIM) scheme, which features a large FOV and fast phase shifting. LF-OS-SIM utilizes a one-dimensional grating to generate stripe patterns and a spatial light modulator (SLM) to perform phase-shifting in the Fourier domain. Therefore, this technique can improve the spatial bandwidth product (SBP) by a factor of 2.6 compared to the conventional OS-SIM under the same experimental parameters. The superiority of LF-OS-SIM is demonstrated by imaging euro coins and fluorescent samples, and the results imply that this method has the potential to be applied for 3D imaging of industrial micro-devices and biosamples.