What question did this study set out to answer?

To determine the molecular mechanism and binding site responsible for interleukin-7-mediated recruitment and activation of phosphatidylinositol 3-kinase.

September 1, 1994European Journal of Immunology

Interleukin‐7 induces the association of phosphatidylinositol 3‐kinase with the α chain of the interleukin‐7 receptor

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Authors

AVAshok R. VenkitaramanAgency for Science, Technology and Research RCRachel J. CowlingCentre National de la Recherche Scientifique

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Implication

In vitro experimental study demonstrates interleukin-7 recruits phosphatidylinositol 3-kinase via phosphorylated tyrosine 449 on its receptor alpha chain, indicating a unique activation mechanism.

Key Points

To determine the molecular mechanism and binding site responsible for interleukin-7-mediated recruitment and activation of phosphatidylinositol 3-kinase.
Assessed interleukin-7 receptor alpha chain tyrosine phosphorylation and association with the p85 regulatory subunit of phosphatidylinositol 3-kinase in the presence and absence of the protein tyrosine kinase inhibitor herbimycin A.
Conducted binding competition assays using phosphopeptides corresponding to alpha-chain cytoplasmic tail tyrosine residues, site-directed mutagenesis (Tyr449Phe), and soluble p85 src homology 2 domain constructs.
Interleukin-7 induced direct association of phosphatidylinositol 3-kinase p85 subunit with the interleukin-7 receptor alpha chain via ligand-dependent phosphorylation of residue Tyr449 within a canonical Tyr(P)-X-X-Met motif.
Binding was specifically blocked by herbimycin A, recombinant p85 src homology 2 domains, a Tyr449-containing phosphopeptide, and replacement of Tyr449 with phenylalanine.

Cite This Study

Venkitaraman et al. (1994) studied this question.

synapsesocial.com/papers/6a715040ce524a4339c4e06b https://doi.org/10.1002/eji.1830240935

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Implication

In vitro experimental study demonstrates interleukin-7 recruits phosphatidylinositol 3-kinase via phosphorylated tyrosine 449 on its receptor alpha chain, indicating a unique activation mechanism.

Interleukin‐7 induces the association of phosphatidylinositol 3‐kinase with the α chain of the interleukin‐7 receptor

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Implication

Key Points

Cite This Study

Interleukin‐7 induces the association of phosphatidylinositol 3‐kinase with the α chain of the interleukin‐7 receptor

Discussion

Member takes

Implication

Key Points

Cite This Study

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