The intensity of staining of each of four classes of neutral lipids (monoacylglycerol, diacylglycerol, triacylglycerol and fatty acid methyl ester) is shown to be dependent on the number or amount of carbon-carbon double bonds (C=C) when using 3% cupric acetate in 8% phosphoric acid. In contrast, staining with 10% cupric sulfate in 8% phosphoric acid is relatively Independent of the number or amount of C=C.
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Baron et al. (1984) studied this question.
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