Regeneration of the connective tissue attachment on surgically exposed roots using a fibrin‐fibronectin adhesive system. An experimental study on the baboon (Papio ursinus)

To evaluate the regeneration of the connective tissue attachment promoted by a fibrin‐fibronectin adhesive system, the buccal, interradicular and interproximal alveolar bone to one half of the length of the roots was removed from the maxillary molars and premolars of 2 chacma baboons. The exposed roots were planed and demineralized with citric acid. On flap closure a fibrin‐fibronectin adhesive system (Tisseel®) was introduced between the root surfaces and the mucoperiosteal flaps in the experimental quadrants. To avoid immunological reactions, Tisseel prepared from baboon plasma was used. Histometrical analysis was performed on a total of 13 experimental and 12 control specimens obtained 95 d post‐operatively. The analysis of variance demonstrated significant differences between the experimental and control specimens with regard to the length of connective tissue attachment or adaptation (p = 0.0234), the extent of newly formed cementum with inserted connective tissue fibers (p = 0.0033) and the amount of bone regeneration (p=0.0068). This limited study indicates that the potential for connective tissue attachment and bone regeneration may be enhanced by the use of specific attachment glycoproteins and plasma factors.