What question did this study set out to answer?

To identify and characterize plant palmitoyl-acyl carrier protein (ACP) thioesterases and determine the evolutionary relationships among plant acyl-ACP thioesterases.

March 1, 1995The Plant Cell

Palmitoyl-Acyl Carrier Protein (ACP) Thioesterase and the Evolutionary Origin of Plant Acyl-ACP Thioesterases

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Authors

AJAubrey JonesNovo Nordisk (United States)HDH. Maelor Davies TVToni A. VoelkerMonsanto (United States)

Discussion

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Implication

Biochemical and phylogenetic analysis reveals the divergence of palmitoyl-ACP thioesterases in plants, highlighting the evolutionary origin of chain-length diversity in fatty acid synthesis.

Key Points

To identify and characterize plant palmitoyl-acyl carrier protein (ACP) thioesterases and determine the evolutionary relationships among plant acyl-ACP thioesterases.
Cloned and characterized cDNAs encoding acyl-ACP thioesterases specific for palmitoyl-ACP (FatB class).
Assayed recombinant enzyme substrate specificity against fatty acyl-ACP substrates of varying chain lengths.
Performed sequence alignments and phylogenetic analyses comparing palmitoyl-ACP thioesterases with known oleoyl-ACP thioesterases (FatA class).
Identified a distinct gene family (FatB) encoding thioesterases with preferential activity toward saturated acyl-ACPs, predominantly 16:0-ACP (palmitoyl-ACP).
Demonstrated that plant acyl-ACP thioesterases comprise two structurally divergent, ancient evolutionary families: FatA (preferring 18:1-oleoyl-ACP) and FatB (preferring saturated acyl-ACPs).
Established that specific structural divergence in these enzymes regulates the chain length of saturated fatty acids exported from plastids.

Cite This Study

Jones et al. (1995) studied this question.

synapsesocial.com/papers/6a715cd06c240de38cdc1c35 https://doi.org/10.2307/3869857

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Implication

Biochemical and phylogenetic analysis reveals the divergence of palmitoyl-ACP thioesterases in plants, highlighting the evolutionary origin of chain-length diversity in fatty acid synthesis.

Palmitoyl-Acyl Carrier Protein (ACP) Thioesterase and the Evolutionary Origin of Plant Acyl-ACP Thioesterases

Discussion

Member takes

Implication

Key Points

Cite This Study

Palmitoyl-Acyl Carrier Protein (ACP) Thioesterase and the Evolutionary Origin of Plant Acyl-ACP Thioesterases

Discussion

Member takes

Implication

Key Points

Cite This Study

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