Successful extraction of DNA from 100‐year‐old herbarium specimens of the liverwort Bazzania trilobata

Using a modified DNeasy™ Plant Mini Kit method to extract DNA, we were able to isolate DNA from 100year‐old liverwort herbarium specimens. We sucessfully isolated DNA from 18 specimens of the liverwort Bazzania trilobata collected during the 20 th century. Our observations suggest that, at least over 100 years of material storage, the most crucial factor in successful DNA isolation is method of drying, not age of sample.