Both arginine and lysine vasopressin have been obtained in a high degree of purity by means of chromatography on carboxymethyl cellulose using ammonium acetate as a volatile buffer and differential elution gradients. Conditions for the resolution of LVP and AVP by chromatography on this same exchanger are described. By combining efficient recovery of activity, high flow rates for the chromatography, and rapid removal of buffers by lyophilization, a simple means of purifying vasopressin is proposed.
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Ward et al. (1957) studied this question.