What question did this study set out to answer?

To determine whether dendritic cell presentation of apoptotic tumor antigens triggers T-cell receptor activation and drives cutaneous T-cell lymphoma cells to adopt an immunosuppressive regulatory phenotype.

October 29, 2004Blood

Cutaneous T-cell lymphoma: malignant proliferation of T-regulatory cells

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Authors

CBCarole L. BergerThe Wistar Institute RTRobert E. TigelaarYale University JCJustine V. CohenHarvard University

Discussion

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Implication

In vitro study reveals dendritic cells induce a regulatory T-cell phenotype in cutaneous T-cell lymphoma, indicating a mechanism for tumor-driven immunosuppression.

Key Points

To determine whether dendritic cell presentation of apoptotic tumor antigens triggers T-cell receptor activation and drives cutaneous T-cell lymphoma cells to adopt an immunosuppressive regulatory phenotype.
Examined CD4+ cutaneous T-cell lymphoma (CTCL) cells in an in vitro model co-cultured with autologous immature dendritic cells loaded with apoptotic CTCL cells or normal CD4+ T cells.
Assessed T-cell receptor activation, calcium mobilization, and the expression of regulatory markers including CD25, CTLA-4, FoxP3, IL-10, and TGF-beta.
Evaluated the requirement of antigen presentation by applying blocking agents against dendritic cell major histocompatibility complex (MHC) class 2 expression and intracellular transport.
Dendritic cells loaded with apoptotic cells stimulated CTCL T-cell receptors, triggering calcium mobilization, membrane down-regulation of CD3/TCR, and up-regulation of CTLA-4.
Stimulated CTCL cells acquired a regulatory T-cell phenotype characterized by FoxP3 expression, secretion of IL-10 and TGF-beta, and suppression of antigen-driven IL-2 and IFN-gamma production in normal T cells.
Blocking dendritic cell MHC class 2 expression or transport effectively inhibited the phenotypic conversion of CTCL cells into regulatory T cells.

Cite This Study

Berger et al. (2004) studied this question.

synapsesocial.com/papers/6a715ece2163a0a01bc58435 https://doi.org/10.1182/blood-2004-06-2181

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October 29, 2004Blood

Cutaneous T-cell lymphoma: malignant proliferation of T-regulatory cells

View Full Paper

Ask AI

Bookmark

Authors

CBCarole L. BergerThe Wistar Institute RTRobert E. TigelaarYale University JCJustine V. CohenHarvard University

Discussion

Member takes

Implication

In vitro study reveals dendritic cells induce a regulatory T-cell phenotype in cutaneous T-cell lymphoma, indicating a mechanism for tumor-driven immunosuppression.

Key Points

To determine whether dendritic cell presentation of apoptotic tumor antigens triggers T-cell receptor activation and drives cutaneous T-cell lymphoma cells to adopt an immunosuppressive regulatory phenotype.
Examined CD4+ cutaneous T-cell lymphoma (CTCL) cells in an in vitro model co-cultured with autologous immature dendritic cells loaded with apoptotic CTCL cells or normal CD4+ T cells.
Assessed T-cell receptor activation, calcium mobilization, and the expression of regulatory markers including CD25, CTLA-4, FoxP3, IL-10, and TGF-beta.
Evaluated the requirement of antigen presentation by applying blocking agents against dendritic cell major histocompatibility complex (MHC) class 2 expression and intracellular transport.
Dendritic cells loaded with apoptotic cells stimulated CTCL T-cell receptors, triggering calcium mobilization, membrane down-regulation of CD3/TCR, and up-regulation of CTLA-4.
Stimulated CTCL cells acquired a regulatory T-cell phenotype characterized by FoxP3 expression, secretion of IL-10 and TGF-beta, and suppression of antigen-driven IL-2 and IFN-gamma production in normal T cells.
Blocking dendritic cell MHC class 2 expression or transport effectively inhibited the phenotypic conversion of CTCL cells into regulatory T cells.

Cite This Study

Berger et al. (2004) studied this question.

synapsesocial.com/papers/6a715ece2163a0a01bc58435 https://doi.org/10.1182/blood-2004-06-2181

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