AGTR1 Inhibits the Progression of Lung Adenocarcinoma

Figures

• 

  Figure 1. Expression level of AGTR1 in different tumors and corresponding adjacent normal tissues. (A) The expression status of the AGTR1 in different cancers or specific cancer subtypes was analyzed through TIMER2. Blue dots indicate the normal tissue and red dots for tumors. (B) Oncomine database indicates that AGTR1 was significantly lower (blue) in tumors compared with corresponding normal tissues. (C) The expression of AGTR1 in ACC and corresponding normal tissues based on the GTEx database. (D) The expression of AGTR1 in OV and corresponding normal tissues based on the GTEx database. (E) The expression of AGTR1 in TGCT and corresponding normal tissues based on the GTEx database. (F) The expression of AGTR1 in UCS and corresponding normal tissues based on the GTEx database. (*P <0 0.05; **P < 0.01; ***P < 0.001).

• 

  Figure 2. Correlation between AGTR1 gene expression and survival prognosis of cancers in TCGA. (A) Kaplan-Meier analysis results based on AGTR1 expression in LUAD, ESCA, KIRC, PAAD, THYM, SARC. (B) Kaplan-Meier analysis results based on AGTR1 expression in BLCA, LUSC, OV, STAD, READ, and UCEC. Red represents high AGTR1 expression, black represents low expression.

• 

  Figure 3. High expression of AGTR1 played a protective role in lung adenocarcinoma based on the GEO database. High AGTR1 expression is associated with a better prognosis in GSE31210 (A), Michigan-LC (B), Jacob-00182-MSK (C), and Jacob-00182-CANDF (D). Red represents high AGTR1 expression, blue represents low expression.

• 

  Figure 4. Correlation analysis between AGTR1 expression and immune infiltration of multiple immune cells. The correlation between AGTR1 expression and the infiltration of B cells (A), DC (B), monocyte (C), MDSC (D) in LUAD. Red represents high immune cells, blue represents low immune cells.

• 

  Figure 5. AGTR1-related genes enrichment analysis. (A) The protein-protein interaction networks of experimentally determined AGTR1-binding proteins. (B) Heat map of the correlation between the top 10 AGTR1-related genes and AGTR1 in pan-cancer. All ten genes were significantly positively related to AGTR1 in lung adenocarcinoma (C) Pathway enrichment analysis of the AGTR1-related genes. (D–F) The top 10 items of GO analysis: biological processes, cellular components, and molecular functions of the AGTR1-related genes.

• 

  Figure 6. Correlation analysis between AGTR1 expression and PI3K/AKT, ERK1/2 pathways. (A) Heat map of the correlation between AGTR1 and the main molecules of PI3K/AKT, ERK1/2 signaling pathway. AGTR1 was positively correlated with AKT3 most PI3K gene subtypes. The KM analysis results of AKT3 (B), PIK3CD (C), PIK3CG (D), PIK3IP1 (E), PIK3R1 (F), PIK3R3 (G), PIK3R5 (H), PIK3R6 (I) in LUAD. Red represents high genes expression, blue represents low expression. (*P <0 0.05; **P < 0.01; ***P < 0.001).

• 

  Figure 7. Validation of role of AGTR1 in LUAD tissues and cells. (A) Immunohistochemical analysis of the expression of AT1R in LUAD and LUAD and benign pulmonary lesions (BPL) samples. (B) The corresponding gray value of immunohistochemical analysis. (C) Survival analysis comparing high and low expression of AT1R in 140 lung adenocarcinoma samples. (D) The mRNA level of AGTR1 in normal lung bronchial cell (BEAS-2B) and lung adenocarcinoma cell lines (NCI-H1299, NCI-H1975, PC9, A549, NCI-H460). (E) The protein level of AT1R in normal lung bronchial cell and lung adenocarcinoma cell lines. (F) The corresponding gray value of western-blot. (D) The results of MTT in 24, 48, and 72 hours. *P<0.05, **P<0.01, ***P<0.001. (G) The percentage of H1299 cells apoptosis in empty and AGTR1 overexpressing group. (H) The mRNA levels of PI3K and AKT3 in a different group. *, #, **represent the comparison of AGTR1, PI3K, and AKT3 between empty and AGTR1 groups, respectively, P<0.05.