Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages.

H L Yin; TP Stossel

doi:10.1016/s0021-9258(19)70589-4

October 1, 1980Journal of Biological ChemistryOpen Access

Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages.

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Key Points

To purify gelsolin from rabbit lung macrophages and determine its physicochemical and calcium-binding characteristics.
Purification of gelsolin from rabbit lung macrophage extracts.
Determination of physical constants including molecular weight, Stokes radius, sedimentation coefficient, and isoelectric point.
Measurement of calcium-binding stoichiometry and affinity in the presence of 0.1 M KCl and 2 mM MgCl2.
Gelsolin is identified as a single polypeptide chain with an average molecular weight of 91,000, a Stokes radius of 44 Å, a sedimentation coefficient (s20,w) of 4.9 S, an isoelectric point of 6.1, and a frictional ratio of 1.43.
The protein accounts for the majority of Ca2+-dependent regulation of actin gel-sol transformation in macrophage extracts.
Gelsolin binds 2 mol of Ca2+ per mole of protein with high affinity (Ka = 1.09 × 10^6 M^-1) under physiological salt conditions.

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Implication

Biochemical analysis reveals structural and calcium-binding properties of gelsolin in rabbit lung macrophages, indicating its primary role in regulating actin gelation.

Abstract

We describe the purification procedure and some of the physiochemical properties of gelsolin, a major Ca2+-dependent regulatory protein of actin gel-sol transformation in rabbit lung macrophages. Gelsolin accounts for the majority of Ca2+ control of actin gelation in macrophage extracts. It is a single polypeptide chain with an average molecular weight of 91,000 a Stokes radius of 44 A, a sedimentation coefficient (s20(0),w) of 4.9 S, an isoelectric point of 6.1, and a frictional ratio of 1.43. Gelsolin binds 2 mol of Ca2+ with high affinity (Ka 1.09 X 10(6) M-1) in the presence of 0.1 M KCl and 2 mM MgCl2.

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Yin et al. (1980) studied this question.

synapsesocial.com/papers/6a7162acac440176ef2a0ffb https://doi.org/10.1016/s0021-9258(19)70589-4

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Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages.

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Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages.

Key Points

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