RNA fractions with messenger activity have been isolated from membrane-bound and free polysomes of rabbit liver. These RNAs were translated in a fractionated, messenger-dependent cell-free system derived from rabbit reticulocytes. The general conditions for translation of these liver RNAs were similar to those for reticulocyte messenger RNA. However, with the liver messenger RNA fractions there was a lower per cent release of labeled polypeptides from the ribosome and a greater requirement for ribosomal wash protein to give half-maximal saturation of messenger RNA translation. Evidence for the synthesis of albumin under the direction of liver messenger RNA was obtained by immunoprecipitation, partial chemical purification, polyacrylamide gel electrophoresis, and gel isoelectric focusing.
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David A. Shafritz (1974) studied this question.
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