Changes in gingival crevicular fluid inflammatory mediator levels during the induction and resolution of experimental gingivitis in humans

Offenbacher S, Barros S, Mendoza L, Mauriello S, Preisser J, Moss K, de Jager M, Aspiras M. Changes in gingival crevicular fluid inflammatory mediator levels during the induction and resolution of experimental gingivitis in humans. J Clin Periodontol 2010; 37: 324–333. doi: 10.1111/j.1600‐051X.2010.01543.x Abstract Aim: The goal of this study is to characterize the changes in 33 biomarkers within the gingival crevicular fluid during the 3‐week induction and 4‐week resolution of stent‐induced, biofilm overgrowth mediated, experimental gingivitis in humans. Methods: Experimental gingivitis was induced in 25 subjects for 21 days followed by treatment with a sonic powered toothbrush for 28 days. Clinical indices and gingival crevicular fluids were collected weekly during induction and biweekly during resolution. Samples were analysed using a bead‐based multiplexing analysis for the simultaneous measurements of 33 biomarkers within each sample including cytokines, matrix‐metalloproteinases (MMPs) and adipokines. Prostaglandin‐E 2 was measured by enzyme‐linked immunoadsorbant assay. Statistical testing using general linear models with structured covariance matrices were performed to compare stent to contralateral (non‐stent) changes in clinical signs and in biomarker levels over time. Results: Gingivitis induction was associated with a significant 2.6‐fold increase in interleukin 1‐ β (IL‐ β ), a 3.1‐fold increase in IL‐1 α and a significant decrease in multiple chemokines as well as MMPs‐1, ‐3 and 13. All changes in clinical signs and mediators rebounded to baseline in response to treatment in the resolution phase. Conclusions: Stent‐induced gingivitis is associated with marked, but reversible increases in IL‐ α a and IL‐1 β with suppression of multiple chemokines as well as selected MMPs.