A method for extraction of extracellular fluid: use in development of a physiological saline for Venus's-flytrap

Extracellular fluid was extracted by centrifugation from the tissue of the traps of the plant Venus's-flytrap. The extracted fluid was subjected to an extensive physicochemical analysis and the results were compared to a similar analysis of exudation sap. The results of the analysis of the extracted fluid served as a basis for the formulation of a physiological perfusion fluid which was compatible with the tissue of the flytrap. The methods used in the extraction and analysis of the fluid are described. The efficacy of the extraction method is discussed.