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Experiments involving ultraviolet irradiation of a tryptophan-independent strain of S. aureus and the exposure of the irradiated cells to penicillin, yielded 10 tryptophan-dependent mutants. Mutants were differentiated by growth responses, syntrophism, and reversion rates. Ten tryptophan-dependent mutants of S. aureus were subjected to a genetic analysis using the transduction technic. This allowed the construction of a chromosome map of 2 different functional linkage groups, closely linked to each other.
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Ritz et al. (1962) studied this question.
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