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January 1, 2014Chemical CommunicationsOpen Access

A chemical “hub” for absolute quantification of a targeted protein: orthogonal integration of elemental and molecular mass spectrometry

Xiaowen Yan Laurent Li Yong Liang Limin Yang Bo Zhang Qiuquan Wang

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Abstract

A novel element-tagged activity-based photo-cleavable biotinylated chemical "Hub" was designed and synthesized to orthogonally integrate ICP-MS and ESI-MS for absolute targeted-protein quantification. This tetrafunctional chemical "hub" allowed us to quantify a targeted protein using species-unspecific isotope dilution ICP-MS and to know which protein is being quantified using ESI-IT-MS at the same time.

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Cite This Study

Yan et al. (2014) studied this question.

synapsesocial.com/papers/6a717d98e36a167817e34dd2 https://doi.org/10.1039/c3cc48460b

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