Answers are grounded in this paper and cite their sources.
were prepared and subjected to forced deamidation by incubation at elevated pH and temperature. Three mutants (G34R, G34K and G34Q) showed increased resistance to deamidation by LC-MS peptide mapping, while maintaining high binding affinity to CD52 antigen measured by Biacore. A complement -dependent cytotoxicity assay indicated that these mutants function by triggering antibody effector function. This study illustrates the importance of structure-based design and extensive mutagenesis to mitigate antibody developability issues.
No takes yet. Share an insight, caveat, or question.
Qiu et al. (2019) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: