A simple method for the determination of catecholamines (norepinephrine, epinephrine, and dopamine) in urine is described. The analytes were extracted from urine by extraction using a monolithic silica disk-packed spin column. The spin column has a phenylboronate moiety, which forms a stable negatively charged complex with cis-hydroxyl groups of catecholamines. The extraction recoveries of catecholamines were above 92.2%. The extracted catecholamines were separated on a reversed-phase column followed by electrochemical detection using a boron-doped diamond electrode. The limits of detection for the catecholamine were 0.2 ng mL-1. The developed method was successfully applied for the determination of catecholamines in urine samples.
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Tsunoda et al. (2011) studied this question.
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