Relationshap between human papillomavirus DNA testing and human telomerase RNA gene amplification detection in esophageal cancer tissue

Objective To study the relationshap between human papillomavirus (HPV) DNA testing and human telomerase (hTERC) RNA gene amplification detection in esophageal cancer tissue.Methods HPV DNA was tested by polymerase chain reaction (PCR) in 82 cases of esophageal cancer tissues.The fluorescence signals of hTERC in the esophageal cancer were detected by using interphase fluorescence in situ hybridization ( FISH ).Results There was HPV infection in esophageal cancer tissue,and the infection rate was about 43.7％.Mixed type was predominant,including the HPV 16 and 18.The mutation rate of hTERC in esophageal cancer tissue was about 80.1％,and there was a positive correlation between the HPV infection and mutation of hTERC (P＜0.05 ).Conclusion HPV infection and mutation of hTERC in the esophageal cancer may be important factors of pathogenesis.The mutation of hTERC gene may plav an important role in the development of sequamous cell carcinoma.