A method for the characterization of sub-milligram quantities of plant coumarins has been devised. Each compound was purified by gas–liquid chromatography, and the collected material analyzed by a combination of ultraviolet absorption and nuclear magnetic resonance spectroscopy. The procedures developed were tested successfully on several plant extracts. Limettin and bergapten were identified in lime peel; angelicin, psoralen, and xanthotoxin in parsnips; and angelicin, xanthotoxin, and bergapten in Angelica leaves. Isopimpinellin was found in all three plant materials.
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Steck et al. (1969) studied this question.