Representative cultures of many bacterial genera were grown in culture media of known composition free of p-aminobenzoic acid. Culture filtrates and hydrolized whole cultures were assayed for p-aminobenzoic acid content employing the Acetobacter suboxydans microbiological method. All organisms successfully cultured in the p-aminobenzoic acid free media elaborated p-aminobenzoic acid in readily measurable amounts, indicating that p-aminobenzoic acid is truly an “essential metabolite” synthesized by many bacteria.
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Landy et al. (1943) studied this question.
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