The performance of particle-loaded membranes (containing 8-µm particles) and traditional packed extraction columns (40-μm particles) are compared. In this study, solid-phase extraction and high-performance liquid chromatographic analysis of the antiarrhythmic drugs, mexiletine and flecainide, are duplicated using two devices that contain chemically similar C8 (octyl) bonded silica. The following parameters are used to assess performance: linearity, recovery precision, elution volume profile, lowest limit of detection, and loading capacity. Overall, the extraction of mexiletine and flecainide from serum using particle-loaded membranes achieves better precision, improved concentrating ability lower detection limits, greater efficiency and reduced solvent volume requirements compared with traditional packed columns. In addition, elution of drugs from the membrane sorbent is efficiently accomplished using a small volume of mobile phase, eliminating analyte instability problems and the need for additional time-consuming evaporation and reconstitution steps.
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Wells et al. (1995) studied this question.