Key result
This review provides best practice guidelines to optimize antibody use for increased rigor and reproducibility in immunoblot analysis and immunohistochemistry approaches.
This review provides best practice guidelines to improve reproducibility and rigor of antibody use in cardiovascular physiology research.
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These guidelines may enhance rigor in cardiovascular protein analyses; leaves open empirical validation before routine lab adoption.
Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches. Listen to this article's corresponding podcast at http://ajpheart.podbean.com/e/guidelines-on-antibody-use-in-physiology-studies/ .
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Brooks et al. (2018) conducted a review in Cardiovascular physiology research. Antibody use guidelines was evaluated. This review provides best practice guidelines to optimize antibody use for increased rigor and reproducibility in immunoblot analysis and immunohistochemistry approaches.
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