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Evaluate novel nutrient media formulations, surfactant additives, and selective chemical screening agents to improve the recovery, enumeration, and identification of cytophagas from aquatic habitats.

December 1, 1972Canadian Journal of Microbiology

The isolation and enumeration of cytophagas

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Evaluate novel nutrient media formulations, surfactant additives, and selective chemical screening agents to improve the recovery, enumeration, and identification of cytophagas from aquatic habitats.
Compared 20 media formulations based on degraded milk protein or tryptone, yeast extract, and sodium acetate against standard media.
Evaluated the effect of adding 0.1 μl/L Tween 20 to dilution blanks versus incorporation into plating agar.
Assayed sensitivity to 0.1% sodium lauryl sulfate (SLS) and proteolytic activity on skim acetate medium across 66 cytophaga strains and 41 non-cytophaga organisms.
Adding 0.1 μl/L Tween 20 to dilution blanks increased cytophaga colony counts up to threefold, whereas adding it directly to plating agar yielded no significant change.
A concentration of 0.1% SLS inhibited growth in 97% of cytophagas (n=66) and in over 80% of non-cytophaga control organisms (n=41).
Combined SLS sensitivity and proteolysis on skim acetate medium occurred in 91% of cytophagas compared to 53% of Flavobacteria and 50% of other tested organisms.

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Implication

Comparative study demonstrates improved enumeration and screening methods for aquatic cytophagas, highlighting a streamlined protocol for identification.

Cite This Study

Christensen et al. (1972) studied this question.

synapsesocial.com/papers/6a71caf526a7f98052ddfa7d https://doi.org/10.1139/m72-299

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Implication

Comparative study demonstrates improved enumeration and screening methods for aquatic cytophagas, highlighting a streamlined protocol for identification.

The isolation and enumeration of cytophagas

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Implication

Cite This Study

The isolation and enumeration of cytophagas

Key Points

Discussion

Implication

Cite This Study

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