Key result
miR-5701 inhibited proliferation and promoted apoptosis of clear cell renal cell carcinoma cells by directly targeting PDE1B.
Why the study?
Because miR-5701 was recently identified as a tumor suppressor in some cancers, its role in clear cell renal cell carcinoma remained to be investigated.
Supports miR-5701 as tumor suppressor in ccRCC models; leaves open clinical translation pending in vivo validation.
Increasing evidence has demonstrated that microRNAs play critical roles in malignant biological behaviors, including cancerogenesis, cancer progression and metastasis, through the regulation of target genes expression. As miR-5701 has recently been identified to play roles as tumor suppressor miRNA in the development of some kinds of cancers, in this study we sought to investigate the role of miR-5701 in clear cell renal cell carcinoma (ccRCC). Colony formation, cell apoptosis and proliferation assays were employed, and the results showed that miR-5701 inhibited proliferation and promoted apoptosis of ccRCC cells. Western blotting and dual-luciferase reporter assays were used to confirm that PDE1B is a new direct target of miR-5701. Furthermore, overexpression of PDE1B attenuated the effects of miR-5701, indicating that miR-5701 inhibited proliferation and promoted apoptosis of ccRCC cells via targeting PDE1B. Taken together, the data presented here indicate that t miR-5701 is a tumor suppressor in ccRCC and PDE1B is a new target of miR-5701.
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Zhao et al. (2021) studied Clear cell renal cell carcinoma (ccRCC). miR-5701 was evaluated on Cell proliferation and apoptosis. miR-5701 inhibited proliferation and promoted apoptosis of clear cell renal cell carcinoma cells by directly targeting PDE1B.
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