Multichain Polyproline Coated with Histidyl and Glutamyl Residues — A Potent Synthetic Immunogen

A new, water soluble multichain polypeptide coated with short peptides containing histidyl and glutamyl residues was prepared, using the 2,4‐dinitro‐phenyl group for masking the imidazole imino‐nitrogen during synthesis. The polymer obtained after thiolytic deblockage (multi‐poly(L‐histidyl, L‐glutamyl)‐poly‐L‐prolyl – – poly‐L‐lysine) was shown to be a potent synthetic immunogen, capable of provoking a high titer of antibodies in mice already at the primary response, and useful for studying the genetic control of the immune response.