What question did this study set out to answer?

This research aims to investigate the activity of angiotensin converting enzyme and angiotensin metabolism in the dog brainstem and spinal cord.

February 1, 1988HypertensionOpen Access

Converting enzyme activity and angiotensin metabolism in the dog brainstem.

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Why the study?

Does ACE inhibition with MK 422 alter the metabolism of Ang I to Ang-(1-7) in dog brainstem homogenates?

Population

Dog brainstem and spinal cord (microdissected regions)

Comparison

In vitro metabolism of 125I-angiotensin I in the… vs Absence of MK 422

Design

Preclinical

Authors

RSRobson A.S. SantosGeneral Cardiology KBK. Bridget BrosnihanAtrium Health Wake Forest Baptist MCMark C. ChappellWake Forest University

Discussion

Member takes

Implication

Highlights potential ACE-independent Ang-(1-7) production in brainstem; leaves open relevance to central autonomic regulation.

Key Points

This research aims to investigate the activity of angiotensin converting enzyme and angiotensin metabolism in the dog brainstem and spinal cord.
Measured concentrations of ACE activity, norepinephrine, and serotonin in microdissected brainstem and spinal cord regions.
Assessed in vitro metabolism of 125I-angiotensin I in homogenates from the same regions.
Evaluated the impact of ACE inhibition using MK 422.
High ACE-specific activity was observed in monoamine-rich areas of the brainstem and intermediolateral column of the spinal cord.
In brainstem homogenates, 125I-Ang I was metabolized primarily to Ang-(1-7) and Ang-(1-8).
Inhibition with MK 422 decreased Ang-(1-8) production while Ang-(1-7) generation persisted.

Structured PICO

Does ACE inhibition with MK 422 alter the metabolism of Ang I to Ang-(1-7) in dog brainstem homogenates?

Population

Dog brainstem and spinal cord (microdissected regions)

Intervention

In vitro metabolism of 125I-angiotensin I (Ang I) in the presence of MK 422 (50 microM, an ACE inhibitor)

Comparator

Absence of MK 422

Outcome

Concentrations of ACE activity, norepinephrine, serotonin, and metabolism of 125I-Ang I to Ang II and Ang-(1-7)surrogate

The study demonstrates high ACE activity in monoamine regions of the dog brainstem and identifies Ang-(1-7) as the major metabolite of Ang I regardless of ACE inhibition.

Cite This Study

Santos et al. (1988) studied this question.

synapsesocial.com/papers/6a71eae38031ec7bb1dd28fa https://doi.org/10.1161/01.hyp.11.2_pt_2.i153

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Converting enzyme activity and angiotensin metabolism in the dog brainstem.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Converting enzyme activity and angiotensin metabolism in the dog brainstem.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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