Detection of some Heavy Metals Using Rhodamine B Isothiocyanate Fluoresceinated Dye

The spectral behavior of the binary complexes of Rhodamine B Isothiocyanate (RBITC) dye with metal ionsin aqueous medium is studied. The observed fluorescence quenching of the dye reveals that the binding constant forRBITC dye-metal ions follow the order Cu2+ > Ni2+ > Pb2+. Also, the spectral changes of the interaction of the studieddye with metal ions in presence of ACES zwitterionic buffer (ternary complexes) is carried out, where the interaction ofdye with ACES zwitterionic buffer is accompanied by as light increasing of the peak intensity at λ= 585 nm and thebinding constant for dye-zwitterionic buffer- metal ion systems were determined where the order of binding with metalions is as follows: Pb2+ > Ni2+ > Cu2+.