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An indirect micro-radioimmunoassay was developed and found to be a highly sensitive and specific method for detecting anti-Newcastle disease virus antibodies in chickens.
An indirect micro-radioimmunoassay is described in which chicken anti-Newcastle disease virus antibody was detected, with radioactively labeled rabbit anti-chicken Fab, on virus-infected microcultures of chick embryo fibroblasts. Newcastle disease virus-infected microcultures were formalin fixed and stored at 4 degrees C for up to 4 months without affecting the sensitivity of the test. The micro-technique was found to be highly sensitive and specific assay of anti-viral antibody and may allow detection of immunoglobulin class of anti-Newcastle disease virus antibody.
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Cleland et al. (1975) studied this question.
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