The efficacy of Ledermix paste in disinfection of dentinal tubules was studied in a model developed by Haapasalo and Orstavik with some modifications. Ledermix and 3% Tetracycline in a hydrous base were effective in reducing the amount of Staphylococcus aureus in dentinal tubules after 7 days of incubation and also after recontamination. They were not effective after 24 h.
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Heling et al. (1991) studied this question.
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