The cultivation of tissues from chick embryos in solutions of NaCl, CaCl₂, KC1 and NaHCO₃

Having found that tissues from chick embryos grow readily in artificial media, nutrient agar and bouillon,l the next step was the attempt to cultivate such tissues in media all the constituents of which are known.In collaboration with Dr. Arthur Koelker we were able to obtain excellent growth in combinations of amidoacids and polypeptids of known constitution, in Locke's solution.Locke's solution with maltose and dextrose was then tried with such good results that Locke's solution was attempted with the very startling discovery that these embryonic tissues grow readily in the Locke's solution and various combinations of NaCl, CaCls, KC1, and NaH CO, as indicated in table 1.So far the cultures in salt solutions have not grown as extensively as those in Locke's solution plus dextrose or maltose or the amido-acids and polypeptids, nor have these given as extensive growth as nutrient agar or bouillon, and these in turn have not been as fine or lived as long as those in plasma.