The Effect of Medium Redox Potential on the Folate-limited Growth of Lactobacillus casei var. rhamnosus

The medium redox potential (Eh) influenced the folate-limited growth of Lactobacillus casei; the growth response was maximal at an Eh of +120 mV (pH 6-35). At raised Eh serum folate would support less growth than pteroylglutamic acid, and the response to N5-methyl tetrahydrofolic acid was intermediate between them. Pteroylglutamic acid was not destroyed during 24 h incubation at 37 degrees C in medium with Eh values between +40 and +440 mV. Destruction of N5-methyl tetrahydrofolic acid occurred within 24 h when the medium Eh was greater than +125 mV. Folate was taken up rapidly by L. casei with an Eh optimum at +270 mV.