Comparative and evaluation of anti-leech (Limnatis Nilotica) effect of Olive (Olea Europaea L.) with Levamisol and Tiabendazole

Until now, there is no registered drug for treatment of complications with leech in the world. According to the available scientific evidence, Olive is an effective anti-parasitic plant. Hence, in the present experiment we studied the inhibitory and killing effect of Olive methanolic extract on Limnatis nilotica. In this study, 100 leeches (Limnatis nilotica) were collected from some wells in western area of Iran (south region in Ilam province) and evaluated the anti-leech effects of Olive methanolic extract (Olea europaea L.) in comparison with levamisole and tinidazole. The results indicated no effect of tinidasole and distilled water on killing or mortality rate of the leeches but Olea europaea L. plant and levamisole have more effect on the L. nilotica. The mean death time of leech for levamisole and Olive determined 10±0.98 and 210±24.1 minutes, respectively. The results showed that treatments of Olive methanolic extract and levamisole have the most effects on leeches and could be used as natural anti-L. nilotica. However it is necessary to achieve further studies for confirm of this subject.