Acid production in milk by Lactobacillus bulgaricus was stimulated by tomato juice or its serum. Preliminary purification of the stimulants involved adsorption and elution on a cation-exchange resin. Unidimensional paper chromatography in two solvent systems was employed in further isolation and purification. Identification of the stimulatory components was based on ultraviolet spectral analysis and thin-layer chromatography. The stimulants were identified as adenine and adenosine.
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Cogan et al. (1968) studied this question.