The rate of spheroplast formation in stationary phase cells of Saccharomyces cerevisiae has been found to be increased as a result of pre-treatment of the cells with protease. The protease has been found selectively to remove the cell wall's mannan-phosphate-protein layer and thus greatly facilitate the action of snail gut enzymes or other such enzymes, in their hydrolytic activity upon the glucan of the cell wall.
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Russell et al. (1973) studied this question.