Acyl type platelet-activating factor in normal rat uterus determined by gas chromatography mass spectrometry

1-Fatty acyl-2-acetyl-sn-glycero-3-phosphocholine (1-fatty acyl-2-acetyl GPC, acyl type platelet-activating factor (PAF) was found in normal rat uterus without addition of any exogenous stimuli and identified as 1-palmitoyl, 1-oleoyl and 1-stearoyl species by gas chromatography/mass spectrometry coupled with selected ion monitoring (SIM). The main species was the 1-palmitoyl species. The ions used for SIM were chosen on the basis of the acetyl and fatty acyl moieties and molecular weight. The amount of 1-fatty acyl-2-acetyl GPC was significantly more than that of PAF (1-fatty alkyl-2-acetyl GPC, AGEPC), and the ratio of 1-palmitoyl-2-acetyl GPC to 1-0-hexadecyl-2-acetyl GPC was about 4.5:1 in normal rat uterus.