Light-scattering and absorption effects caused by addition of adenosine diphosphate to rat-heart-muscle sarcosomes

EFFECTS OF ADP IN chrome b, and two explanations for his result are considered.5.The cross-over point for the effect of addition of adenosine diphosphate upon the steady-state oxidation-reduction level of the cytochromes lies between b and c.This result is consistent with observations on mitochondrial preparations and indicates the similarity of the electron-transfer and phosphorylation mechanisms in heart sarcosomes and in liver mitochondria.6.The observations suggest that the response of the oxidation-reduction levels of the components of the respiratory chain to increases and decreases of concentration of adenosine diphosphate is a basic characteristic of the electron-transfer and phosphorylation mechanisn and is to be found in sarcosomes and mitochondria of many cell types.