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Recently, it has been demonstrated that cryo-focused ion beam milling (cryo-FIB) can be used to obtain high-quality samples from complex cellular environments for cryo-electron tomography (cryo-ET) from both plunge-frozen and high pressure frozen (HPF) cells, organisms and tissues [1-3]. These advances revealed the inner organization of cells and tissues at the molecular scale and at unprecedented resolution, coining the term “molecular sociology” [4].
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Plitzko et al. (2021) studied this question.
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