N‐Methylation of Isopeptide Bond as a Strategy to Resist Deubiquitinases

Stable like a rock: An efficient method to generate N-methylated isopeptide bonds has been developed. The strategy was used to generate highly stable ubiquitinated peptides and proteins that are resistant to deubiquitinases (DUBs; see scheme). Thus, the behavior of several stable ubiquitin conjugates with different DUBs was studied in vitro and within a cellular environment.