The ability of metal salts and pharmacological agents to induce oxidative stress and the SOS response in bacteria is tested with a set of lux biosensors. The sensors are based on three E. coli strains carrying recombinant plasmids with the lux operon fused to the promoters of the SoxS (superoxide dismutase), KatG (catalase–peroxidase), and ColD (colicin D) genes. A total of 47 substances are tested. Of them, 16 induce the SOS response; and 6, oxidative stress. The results observed are compared with the previously published data on the mutagenic activity of the same 47 substances evaluated in the Ames test. The comparison shows full coincidence for 42 of the 47 substances analyzed. The potential of lux-based biosensors for screening chemicals for genetic activity is discussed.
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Игонина et al. (2018) studied this question.
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