In order to study the possible function of plastoquinone in the photosynthetic electron transport system, a method for extracting the endogenous quinone from isolated chloroplasts and measuring its concentration and oxidation-reduction state has been devised. Using this method it has been shown that the plastoquinone in freshly isolated chloroplasts is predominantly in the oxidized form. The concentration of plastoquinone was 0·1 mole per mole chlorophyll (mean value). Comparison of this concentration with that of the published concentration of cytochrome/indicated that plastoquinone was present in a considerable excess on a molar basis.
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Redfearn et al. (1962) studied this question.
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